Technician – Protein Production
Technician role with a background in molecular biology or biochemistry techniques, able to conduct protein expression in E. coli, protein purification using different strategies, and molecular cloning
We usually respond within a week
About Us
Portal Biotech is a pioneering biotechnology company dedicated to developing innovative solutions for proteomics. Our team consists of passionate scientists, engineers, and professionals driven by a shared mission to make a meaningful impact on human health and biology.
About the role:
We are currently seeking a talented and motivated Technician with a background in molecular biology or biochemistry techniques to join our dynamic team. The selected candidate will play a key role in our research and development efforts, contributing to the discovery and development of novel technologies in proteomics and metabolomics. As a technician you will be responsible for conducting protein expression in E. coli, protein purification using different strategies, and molecular cloning. As part of our dynamic research team, you will contribute to the development of multiple projects, gaining hands-on experience in cutting-edge techniques while working in a collaborative and innovative environment.
Responsibilities:
- Perform heterologous protein expression in Escherichia coli using various expression systems.
- Purify proteins using different techniques such as affinity chromatography (His-tag, GST-tag, etc.), ion exchange, size exclusion chromatography (SEC), and other methods.
- Carry out molecular cloning, including PCR, restriction digestion, ligation, and transformation.
- Analyse protein samples using SDS-PAGE, and spectrophotometric assays.
- Maintain accurate and detailed laboratory records in compliance with standard operating procedures (SOP and good laboratory practices (GLP).
- Assist in troubleshooting and optimizing expression and purification protocols.
- Support lab organization, including reagent preparation, equipment maintenance, and inventory management.
- Collaborate with researchers, sharing findings and contributing to project discussions.
Requirements:
- Bachelor’s or Master’s degree in Biochemistry, Molecular Biology, Biotechnology, or a related field.
- Hands-on experience in protein expression and purification.
- Experience with FPLC (ÄKTA system) or other automated purification systems is preferred.
- Familiarity with molecular biology techniques, especially cloning, PCR, and plasmid preparation.
- Strong analytical and problem-solving skills.
- Ability to follow standard protocols and maintain accurate documentation.
- Strong organisational skills and attention to detail.
Portal is an equal opportunities employer. We are enriched by our diversity and are committed to creating and sustaining an inclusive and equitable environment for all. We are a values-led company, and the dedication, passion, and enthusiasm of our talented employees is our cornerstone. We strive to create an environment where our team feels empowered to be their best selves, and where they enjoy working and building both the technologies and our company with us.
We encourage applications from candidates of all backgrounds and experiences.
- Team
- Biology R&D
- Role
- Technician – Protein Production
- Locations
- Groningen
About Portal Biotech
Founded by DNA-sequencing veterans, Portal Biotech is developing the first bench-top single-molecule protein sequencer, leveraging machine-learning algorithms and building on decades of in-house expertise in nanopore technology. By analysing full-length protein molecules at the single-molecule level, our platform delivers rapid, real-time information on protein identity, abundance, and structure. Those insights open frontiers in drug discovery, diagnostics, and fundamental research, helping scientists and clinicians to better understand human health and disease.
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